SMYD3 antibody (N-Term)

Catalog No. ABIN6265199

This Rabbit Polyclonal antibody specifically detects SMYD3 in WB, IHC, IF and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for SMYD3 antibody (N-Term) (ABIN6265199)

Target: SMYD3 (SET and MYND Domain Containing 3 (SMYD3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMYD3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-SMYD3 Antibody

Binding Specificity: N-Term

Specificity: SMYD3 Antibody detects endogenous levels of total SMYD3.

Predicted Reactivity: Pig,Zebrafish,Bovine

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human SMYD3, corresponding to a region within N-terminal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for SMYD3

Target: SMYD3 (SET and MYND Domain Containing 3 (SMYD3))

Alternative Name: SMYD3

Background:

Description: Histone methyltransferase. Specifically methylates 'Lys-4' of histone H3, inducing di- and tri-methylation, but not monomethylation (PubMed:15235609, PubMed:22419068). Also methylates 'Lys-5' of histone H4 (PubMed:22419068). Plays an important role in transcriptional activation as a member of an RNA polymerase complex (PubMed:15235609). Binds DNA containing 5'-CCCTCC-3' or 5'-GAGGGG-3' sequences (PubMed:15235609).

Gene: SMYD3

Molecular Weight: 42-45 kDa

Gene ID: 64754

UniProt: Q9H7B4